hek 293 t cells (ATCC)

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ATCC hek 293 t cells
Hek 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek+293+t+cells/293T/pm42236761-139-1-5
Average 99 stars, based on 37945 article reviews

hek 293 t cells - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: Rational design of a modular mRNA vaccine platform for rapid adaptation to SARS-CoV-2 variants.
Article Snippet: In vitro transfection of mRNA was conducted in HEK 293 T cells using Lipofectamine® 3000 reagent (Invitrogen, USA) following the manufacturer’s instructions. .. Briefly, HEK 293 T cells (ATCC, CRL-3216TM) were cultured in DMEM (Gibco, USA) containing 10% FBS (Gibco, USA). .. Cell detachment was achieved using 0.25% TrypLE (Thermo Fisher Scientific, USA), and cells were seeded in 24-well plates at a density of 2.5 × 105 cells in 500 μl DMEM medium containing 2% FBS.

Article Title: Directed evolution of APOX for proximity labeling using phenols with high redox potentials.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken α-c-Myc MilliporeSigma Cat#AB3252 Goat AlexaFluor647-αCh ThermoFisher Cat#A-21449 Streptavidin-Phycoerythrin (SA-PE) ThermoFisher Cat#S886 Streptavidin-AlexaFluor568 (SA-A568) ThermoFisher Cat#S11226 Streptavidin-HRP (SA-HRP) ThermoFisher Cat#S911 Rabbit-αFlag Sigma Cat#F7425 Goat AlexaFluor488-αRabbit ThermoFisher Cat#A11008 mouse anti-BSA Sigma Cat#SAB4200688-100UL goat anti-mouse HRP conjugate Sigma Cat#AP124P Streptavidin-AlexaFluor594 (SA-A594) Invitrogen Cat#S11227 Bacterial and virus strains DH5α E.coli Invitrogen Cat#12034013 Saccharomyces cerevisiae strain BJ5465 ATCC Cat#208289 Biological samples Cortical neuronal cultures from prenatal Sprague-Dawley rats Envigo https://www.inotiv.com/hubfs/resources/ data-sheets/envigo-44-sprague-dawleymodel-sheet-cardiology-a4_screen.pdf Chemicals, peptides, and recombinant proteins Biotin-PEG3-azide ThermoFisher Cat#50-210-7600 Desthiobiotin-PEG3-azide Sigma Cat#902020 200 μL PierceTM Streptavidin Agarose beads Thermo Cat#20349 Carbonic anhydrase from bovine erythrocytes Sigma Cat#C3934-100MG Horseradish peroxidase Biosynth Cat#FP159434 phenosafranin Sigma Cat#199648-1G Nile blue Sigma Cat#370088 Critical commercial assays Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat#D2004 Deposited data mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE partner repository PXD060753 and 10.6019/PXD060753 https://www.ebi.ac.uk/pride/archive/ projects/PXD060753 Experimental models: Cell lines HEK 293 T ATCC CRL-3216 Recombinant DNA FUGW 3rd generation lentiviral plasmid Lois et al.81 Addgene plasmid #14883 Oligonucleotides Con2.Fwd (5′-CTAGTGGTGGAGGAGGCTCTGG TGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAG-3′) Integrated DNA Technologies N/A Con2.Rev (5′-TATCAGATCTCGAGCTATTACAAGTCCT CTTCAGAAATAAGCTTTTGTTCGGATCC-3′) Integrated DNA Technologies N/A HomR.Fwd (5′-CAAGGTCTGCAGGCTAGTGG TGGAGGAGGCTCTGGTG-3′) Integrated DNA Technologies N/A HomR.Rev (5′-CTACACTGTTGTTATCAGATC TCGAGCTATTACAAGTC-3′) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS Technology e1 Cell Chemical Biology 33, 1–15.e1–e13, April 16, 2026 .. HEK 293 T cells from ATCC (CRL-3216, passage number < 25, female origin, authenticated using STR profiling and mycoplasma testing) were cultured as a monolayer in DMEM (ATCC) supplemented with 10% (v/v) FBS (Corning) and 2 mM L-glutamine (ATCC) (DMEM-FBS) at 37 ◦C under 5% CO2. .. For fluorescence imaging experiments, cells were grown on Costar® 48-well Clear TC-treated Multiple Well Plates (Corning 3548).

Incubation:

Article Title: Mechanistic insights into the therapeutic properties of delta opioid receptor
Article Snippet: The results were analyzed using one-site models with GraphPad Prism 10.1.1 software using one-site model. .. A mixture of the receptor of interest (300 ng per 6-well) and TransIT 2020 (1 ng:2 μl ratio, Mirus Bio) in Opti-MEM was incubated for 30 min and added to HEK-293 T cells (ATCC) for transfection. ..

Article Title: Mechanistic insights into the therapeutic properties of delta opioid receptor
Article Snippet: .. To prepare the membranes, a mixture of the receptor of interest (20 μg per 15-cm dish) and TransIT 2020 (1 ng:2 μl ratio, Mirus Bio) in Opti-MEM was incubated for 30 min and added to HEK-293 T cells (ATCC) for transfection. ..

Transfection:

Article Title: Mechanistic insights into the therapeutic properties of delta opioid receptor
Article Snippet: .. HEK-293 T cells [American Type Culture Collection (ATCC)] were transfected with a mixture of receptor and G proteins (Gα-Rluc, Gβ, and Gγ-GFP triple construct) in a 1:5 ratio (300 ng of receptor and 500 ng of G proteins for 10-cm dishes) unless otherwise noted. ..

Article Title: Mesenchymal stem cell exosomes alleviate ischemic myocardial injury by miR-125b-5p/BTG2 pathway.
Article Snippet: 1 Department of Cardiology, Fujian Cardiovascular Medical Center, Fujian Institute of Coronary Artery Disease, Fujian Medical University Union Hospital, Fuzhou 350001, China 2 Fujian Provincial Clinical Research Center for Cardiovascular Diseases Heart Center of Fujian Medical University, Fuzhou, China 3 School of Union Clinical Health, Fujian Medical University, Fuzhou, China Abstract MicroRNAs mediate the protective effects of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) against myocardial injury.. This study aimed to elucidate the specific role of exosomal miR-125b-5p in ischemic myocardial injury, focusing on its regulatory interaction with B-cell translocation gene 2 (BTG2).. Murine BMSCs were transfected with miR-125b-5p inhibitor or negative-control (NC) oligonucleotides and then used to generate miR-125b-5p-knockdown (miR-125b-5pKD)-Exos or NC-Exos under hypoxic condition.

Generated:

Article Title: Humoral immunity and infection status of PLWH following vaccination after the BA.5/BF.7 wave
Article Snippet: The cells were disrupted at confluence with 0.25% trypsin in 1 mM EDTA (Solarbio, China) every 48–72 h. .. Pseudovirus particles were generated as previously described by cotransfecting HEK-293 T cells (ATCC, CRL-3216) with human immunodeficiency virus backbones expressing firefly luciferase (pNL4-3-R-E-luciferase) and the pcDNA3.1 vector encoding the S protein of the D614G, BA.5, and XBB.1.5 plasmids. .. Codon-optimized, full-length open reading frames of the spike genes of the D614G, BA.5, and XBB.1.5 strains were synthesized by GenScript (Nanjing, China).

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Article Title: Proteasomal degradation of intracellularly expressed Amblyomin-X limits suicide gene therapy potential in melanoma cells.
Article Snippet: .. The cells used in this study were SK-MEL-28 (RRID: CVCL_0526, ATCC, USA), human melanocytes derived from a patient with malignant melanoma, and HEK-293 T cells (RRID: CVCL_0063, ATCC, USA), nontumoral human epithelial-like cells isolated from the kidney of a fetus. .. All cells were cultured in flasks in an incubator at 37 °C with 5% CO2, using Dulbecco’s modified Eagle medium High Glucose (DMEM, Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, USA) and 1% PenStrep antibiotics (Gibco, USA).

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